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Fish Health Section
American Fisheries Society
Real-time RT-PCR Theory & Practice
sponsored by
R OC H E D i a g n os t i c s C a n a d a
Theory and Practice of Real-time RT-PCR for Virus Detection and Quantitation. Postconference workshop offered by the OIE Reference Lab for ISA, limited to 10 people (first
come basis) and will concentrate on Virus detection and quantification methodologies.
This workshop will be held on Saturday afternoon, 13 July 2008 and will run for 3-4 hours,
and will be conducted by Dr. Fred Kibenge, Atlantic Veterinary College.
WORKSHOP OUTLINE
1.
Introduction to real-time RT-PCR
2.
Principle [amplification plot; conventional (end-point) RT-PCR versus real-time
RT-PCR]
3.
Real-time Chemistries (Methods of detection) and targets including melting curve
analysis.
4.
Quantification in real-time RT-PCR (Standard curve; Absolute Quantitation;
Relative Quantitation; Comparative Quantitation)
5.
Factors, Formats and Controls (including a discussion on RNA integrity; Reverse
transcriptases; use and choice of house keeping genes)
6.
Real-time PCR Systems (Instruments)
7.
Data analysis
8.
Reports.
FHS / AFS 2008 Annual Meeting
Charlottetown, Prince Edward Island, Canada
Phone: 902-566-0864 Fax: 902-566-0723 email: [email protected]
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