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A
SalI
SphI
ori
HindIII
pBlueIIKS+-GAP repair
rescue construct
F24o1
STM
PPR
564bp
479bp
C
Restriction digestion analysis of independent GAP
repair recombinations into the pBlueIIKS+
construct.
GAP-repair HR
B
M1 1
2
M1
S a l I (17508)
3.0
2.0
6.0
10.0
6.0
E c o RV (20450)
E c o RV (17243)
Hin dIII (2339)
Hin dIII (17007)
kb
48.5
38.4
29.9
24.6
19.4
15.0
10.0
kb
H in dIII (1)
3
Hin dIII (16272)
M2 M1 1
2
3
3.0
Pm e I (15999)
Hin dIII (15634)
p B lueAtST M 1 7
Hin dIII (15535)
20453 bp
Predicted PPR (14226 - 15676)
Hin dIII (13932)
EcoRV digests.
Standard gel results
Hin dIII (13565)
STM coding sequence (10439 - 13365)
Hin dIII (11453)
E c o RV (11049)
PmeI digests.
FIGE results
Lane 3 shows the restriction patterns as expected from the
correct recombination event in pBlueAtSTM17 as in B
1/--страниц
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